Fig. 1: SARS-CoV-2 501Y.V2 is resistant to monoclonal antibodies.

a, Structure of SARS-CoV-2 RBD (yellow) modeled in complex with class 1 (translucent green) or class 2 (translucent purple) neutralizing antibodies. Side chains of residues K417, E484 and N501 are indicated. mAb, monoclonal antibody. b, A plot showing percentage of K417 accessible surface area (x axis) buried (buried surface area) in class 1 antibody paratopes (listed on the y axis). VH3-53/66 antibodies are separated below the horizontal line. c, ELISA binding for CA1, LyCoV016 and CC12.1 to the original (black) or the 501Y.V2 RBD (red). d, Neutralization curves for the same antibodies shown in c, against the original pseudovirus (black), 501Y.V2 (red) or a chimeric construct that includes only the RBD substitutions K417N, E484K and N501Y (orange). e, Percentage of E484 accessible surface area buried in class 2 antibody paratopes (listed on y axis). VH1-2 antibodies (middle) or sy-/nanobodies (bottom) are separated with horizontal lines. f, ELISA binding for BD23, C119 and P2B-2F6 to the original (black) or 501Y.V2 RBD (red). g, Neutralization curves for the same antibodies shown in f, against original (black), 501Y.V2 (red) or RBD chimeric pseudoviruses (orange). h, Structure of SARS-CoV-2 NTD (cyan) modeled in complex with VH1-24 neutralizing antibody (translucent maroon). The N5-loop supersite and residue R246 are indicated. i, Contribution of N5 loop and R246 to NTD-directed neutralizing antibodies is indicated. j, Modeling of the Δ242-244 deletion (lime green). NTD loops N1, N3 and N5 are shown and the position of R246 in the original NTD and 501Y.V2 NTD is labeled with black and pink, respectively. The minimum displacement for 501Y.V2 loop N5 and the accompanying clash with R102 are indicated with pink arrows. k, ELISA binding for 4A8 to original (black) or 501Y.V2 NTD (red). l, Neutralization curves for 4A8 against the original (black) or 501Y.V2 (red) pseudovirus. All experiments were performed in duplicate.