Fig. 3: Antibody binding to cells expressing D614G, B.1.1.7 and B.1.351 S proteins.
From: Sensitivity of infectious SARS-CoV-2 B.1.1.7 and B.1.351 variants to neutralizing antibodies
a, Binding of monoclonal antibodies or soluble ACE2. HEK293T cells were transiently transfected with plasmids expressing the D614G, B.1.1.7 and B.1.351 S proteins. After 24 h, cells were stained with SARS-CoV-2 antibody mAb10 (a pan-coronavirus antibody), mAb48, mAb102 or soluble ACE2 (ACE2-biotin at 10 μg ml−1 revealed with fluorescent streptavidin) and analyzed by flow cytometry. One representative example of binding is shown. b, Titration binding curves of mAb48, mAb102 and ACE2 to the three S proteins. Data are the mean ± s.d. of three independent experiments. c, Binding of the panel of 83 sera from 58 convalescent individuals. Sera were tested at a 1:300 dilution. Data are the mean of two independent experiments. Two-sided Friedman test with Dunn’s test for multiple comparisons was performed between each viral strain at the different time points, *P < 0.05. M6: B.1.1.7 versus B.1.351, P = 0.037.
