Extended Data Fig. 8: Single-cell BCR clonotype expansion analysis across COVID-19 severity.
From: Single-cell multi-omics analysis of the immune response in COVID-19
a, UMAP visualisation of B cell lineage and coloured by clonotype size in the V(D)J data. Only expanded clonotypes are coloured (clonotype size > 2). b, Single-cell BCR network plots for each severity status coloured by assigned cell type. c, Single-cell BCR network plots for each severity status coloured by heavy chain isotype subclass (IgM, IgD, IgA1, IgA2, IgE, IgG1, IgG2, IgG3 or IgG4). Each circle/node corresponds to a single B cell with a corresponding set of BCR(s). Each clonotype is presented as a minimally connected graph with edge widths scaled to 1/d + 1 for edge weight d where d corresponds to the total (Levenshtein) edit distance of BCRs between two cells. Size of nodes is scaled according to increasing node closeness centrality scores that is nodes that are highly central to a clonotype network will be larger.
