Extended Data Fig. 9: Gaussian Mixture Modelling of CyCIF data.
From: Evolution of delayed resistance to immunotherapy in a melanoma responder
a, Heatmap demonstrating clusters of cells from Gaussian Mixture Model clustering characterized by a range of CyCIF quantitative fluorescence (Methods) from the 19 tumors in Batch 1. b, Heatmap demonstrating clusters of cells from Gaussian Mixture Model clustering characterized by a range of CyCIF quantitative fluorescence (Methods) from the pre- and post-TLR9 + antiPD1 therapy tumors (Batch 2). (Top) There is a distinct NGFR-Hi tumor cell cluster, which is high in PD-L1 protein expression, a MITF-Hi/NGFR-lo tumor cell cluster, and an immune cell cluster. Several non-specific (that is non-NGFR-Hi, non-MITF-Hi) tumor cell clusters are also seen. (Bottom) There is a strong association between NGFR and PD-L1 expression among S100 + gated tumor cells (Pearson correlation coefficient r = 0.66 and p-value = 0, calculated by the default function in MATLAB).
