Extended Data Fig. 6: NASH boosts DC-poiesis.
From: XCR1+ type 1 conventional dendritic cells drive liver pathology in non-alcoholic steatohepatitis
a, Flow cytometry plots illustrating gating strategy for assessing BrdU incorporation in MDP, CDPs and preDCs in the bone marrow, blood and liver. b, Percentage of BrdU+ cells among MDP in the bone marrow of mice fed ND or MCDD for two weeks and pulsed with BrdU 10 h prior to DC progenitor analysis. n = 3 per group, data are presented as mean ± s.e.m. c, kNN graph of bone marrow-isolated CDP, pre-DC isolated form the bone marrow, blood and liver and liver DC isolated from wild-type mice kept on ND or fed MCDD for two weeks. d, Plot representing expression of marker genes in annotated clusters in cells showed in c. Color intensity indicates log2 of the mean UMI count; circle size represents percentage of cells within cluster expressing indicated genes. e, kNN graphs showing distribution of single cells from different organs and diet-regiments matched with bar plots showing relative contribution of each annotated cell type among CDP sorted from the bone marrow and pre-DCs sorted from the bone marrow, blood and liver of wild-type mice kept on ND or on MCDD for two weeks. Plots were down-sampled to 680 cells for CDP, 1404 for bone marrow preDC, 601 for blood preDC and 622 for liver preDC.
