Extended Data Fig. 7: Characterization of genetic signatures of DC-T pairs in liver lymph nodes in NASH.
From: XCR1+ type 1 conventional dendritic cells drive liver pathology in non-alcoholic steatohepatitis
a, Full gating strategy for T and DC, as well as PIC-sorting from murine liver lymph nodes. b, Number of reads, number of UMIs and percentage of cells analyzed per batch of 380 cells (that were pooled for library construction) in PIC-seq experiments. c, Heat map of differential gene expression for all single cells sorted as DC and T cells, annotated based on their gene expression, and used to create ‘expected PICs’ for PIC-seq analysis. Highlighted are marker genes for the annotated DC and T subtypes. d,e, Gene-expression levels in observed PICs assigned to the cDC1-T identity (d) or cDC2-T identity (e), plotted against their expected levels as determined by PICseq in the liver lymph nodes of mice kept on ND or MCDD for two weeks. Highlighted genes are expressed differentially between observed and expected PICs in one or both conditions.
