Extended Data Fig. 10: cDC1 modulate hepatic immune cell populations in the MCDD model of NASH.

a,b, Representative flow cytometry plots (a) and flow cytometry-based quantification (b) of CD8 + cells in the livers of control and Xcr1DTA mice kept on ND or MCDD for two weeks. n = 4 per group. c,d, Representative microscopic images of IHC staining for CD8 (c), and image-based quantification (d) of the stained CD8 + cells in the livers of control and Xcr1DTA mice kept on ND or MCDD for two weeks. bar=100 μm. For quantification 5–10 randomly selected fields per mouse were averaged, n = 5 mice in control and Xcr1DTA-ND groups, n = 4 in the Xcr1DTA-MCDD group. e, FACS-based quantification of CD44 + CD62L + T central memory CD8 + T cells and naïve CD44-CD8 + T cells, separated as shown in (a) in the livers of control and Xcr1DTA mice kept on ND or MCDD for two weeks. n = 4 individual mice per group f, Flow cytometry plots representing gating strategy used to dissect CD4 + T cell subpopulations. g, Flow cytometry-based quantification of CD4 + T cell percentage among CD45 + immune cells in the livers of control and Xcr1DTA mice kept on ND or MCDD for two weeks. n = 4 mice per group h,i, Representative microscopic images of IHC staining for CD4 (h) and image based quantification (i) of CD4 + cells in the livers of control and Xcr1DTA mice kept on ND or MCDD for two weeks. bar = 100 μm. For quantification 5–10 randomly selected fields per mouse were averaged, n = 5 mice per group. j, Flow cytometry-based quantification of CD4 + T cell subsets in the livers of control and Xcr1DTA mice kept on ND or MCDD for two weeks. n = 4 mice per group k,l, Flow cytometry contour plots and flow cytometry-based quantification of NKT cells (k) and monocytes (l) in the livers of control and Xcr1DTA mice kept on ND or MCDD for two weeks. n = 4 mice per group m, Representative microscopic images of IHC staining for CD42b, a marker of platelets, and image based quantification of the stained cells in the livers of control and Xcr1DTA mice kept on ND or MCDD for two weeks. bar = 100 μm. For quantification 5–10 randomly selected fields per mouse were averaged, n = 5 mice per group. In all graphs data are presented as mean ± s.e.m. P-values were determined by one-way ANOVA with Tukey’s multiple comparisons test; ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.