Extended Data Fig. 3: Hepatic cDC increase in NASH.
From: XCR1+ type 1 conventional dendritic cells drive liver pathology in non-alcoholic steatohepatitis
a, Flow cytometry plots illustrating gating strategy for cDCs, cDC1, cDC2 and mDC quantification and sorting. b, Heat map of differential gene expression of cDC sorted from Xcr1Cre-mTFP1 reporter mice. Top plots illustrate relative fluorescent intensity of indicated FACS markers in each cell, ordered as in the heat map. c, Representative flow cytometry contour-plots of CD11c + MHC-II + cells out of CD45 + Lin- gate in livers of mice fed ND or MCDD for two weeks. Population frequencies represent mean ± s.e.m. d, Representative immunofluorescence images of CD11c (red), MHC-II (green) and CD31 (vasculature marker; magenta) in liver section of mice fed ND or MCDD for two weeks. Cell nuclei are stained with DAPI (blue). Scale bar, 30 μm. Images representative for two independent experiments. e, Representative pictures of H&E staining of livers in wild type C57/bl mice fed with ND or WD for 3 and 6 months. f, Percentage of CD11c + MHC-II + DCs among CD45 + cells in the livers of mice fed WD for 3 months, n = 6 per group, data are presented as mean ± s.e.m.; p-value was determined by two-tailed Student’s t-test. g, Representative pictures of H&E staining of livers in wild type C57/bl mice fed with ND or CDHFD for 6 months.
