Extended Data Fig. 7: Immune markers of CICB toxicity.
From: Gut microbiota signatures are associated with toxicity to combined CTLA-4 and PD-1 blockade
a, b, Comparison of Ki67+ cells within CD8+ T effectors (Teff; a) and T central memory (TCM; b) cells in early on-treatment blood samples between patients with available blood samples (n = 14) grouped according to high-grade irAE (Mann-Whitney test left panel p = 0.0044, right panel p = 0.013). c, Gating strategy for key CD4/8+ T cell populations. d, e, Percentage of CD28+ cells within CD4+ Teff (c) and CD27+ cells within CD8+ Teff (d) measured at baseline in this patient cohort (MDACC; left panels) and a separate cohort of patients treated with CICB at Memorial Sloan-Kettering Cancer Center (MSKCC; right panels). Data are grouped by experience of high-grade irAE (Mann-Whitney test (d) left panel p = 0.014, right panel p = 0.050 (e) left panel p = 0.072, right panel p = 0.32)). f, Boxplot depicting a higher diversity of the peripheral T cell repertoire as measured by TCR Vβ sequencing in patients experiencing high-grade irAE (n = 24, Mann-Whitney test; p = 0.028). g, Boxplot showing the number of significantly expanded T cell clones (pre- to on-treatment) detected by TCR sequencing of the peripheral blood immune repertoire, grouped by presence or absence of high-grade irAE (n = 16, Mann-Whitney test: p = 0.22). Box plots present the median bar with the box bounding interquartile range (IQR) and whiskers to the most extreme point within 1.5 x IQR. All tests are two sided unless otherwise specified.
