Extended Data Fig. 5: PfSPZ Vaccine-induced T cell responses. | Nature Medicine

Extended Data Fig. 5: PfSPZ Vaccine-induced T cell responses.

From: Safety, immunogenicity and efficacy of PfSPZ Vaccine against malaria in infants in western Kenya: a double-blind, randomized, placebo-controlled phase 2 trial

Extended Data Fig. 5

(a–d) T cell response to PfSPZ Vaccine. Percent of non-naïve CD4 T cells in the blood expressing IL-8 (A), IL-21 (b), Th2 cytokines (c), or IL-17 (d) at pre-immunization (blue) or 2 weeks after the 3rd immunization (red) of the indicated dose of PfSPZ Vaccine. Results are the percentage of cytokine-producing cells after incubation with PfSPZ minus the percentage of cytokine-producing cells after incubation with vaccine diluent (medium with 1% human serum albumin). Bars indicate median values within each group. Bars indicate median values and differences at each timepoint between pre and post vaccination groups were assessed using multiple T tests with Holm-Sidak’s correction for multiple comparisons. (e) A single PfSPZ-vaccinated adult PBMC sample was included in each batch as a positive control for PfSPZ stimulation. Each symbol represents the percent of non-naïve CD4 T cells expressing CD154, IFNγ, IL-2, or TNF (blue) or γδ T cells expressing IFNγ, IL-2, or TNF (red) in the positive control sample within a single batch. Green bars indicate median values. (f) Percent of non-naïve CD4 T cells expressing CD154, IFNγ, IL-2, or TNF or CD8 T cells expressing IFNγ, IL-2, or TNF in adult control samples following PMA/ionomycin stimulation.

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