Extended Data Fig. 4: Transduction of CD34 + cells with SAMD9/9L mutant lentiviruses. | Nature Medicine

Extended Data Fig. 4: Transduction of CD34 + cells with SAMD9/9L mutant lentiviruses.

From: Clinical evolution, genetic landscape and trajectories of clonal hematopoiesis in SAMD9/SAMD9L syndromes

Extended Data Fig. 4

(a) RT-PCR confirming the overexpression of SAMD9 wildtype, SAMD9 E974K, SAMD9L wildtype and SAMD9L V1512M lentiviruses compared to empty control in sorted GFP positive CD34 + cells 24 h post transduction. Mean ± SEM from n = 2 independent experiments are presented. SAMD9 and SAMD9L expression data is normalized to GAPDH, and untransduced CD34 + sample is set to 1. (b) Flow cytometry plots outlining staining strategy with Annexin V-APC and DAPI in GFP positive CD34 + cells transduced with lentiviral constructs (top: SAMD9L, bottom SAMD9). Corresponding data are shown in main manuscript Fig. 3d.

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