Extended Data Fig. 5: Mass cytometry characterizes the effector-memory phenotype of CAR-T cells over time. | Nature Medicine

Extended Data Fig. 5: Mass cytometry characterizes the effector-memory phenotype of CAR-T cells over time.

From: Neurocognitive and hypokinetic movement disorder with features of parkinsonism after BCMA-targeting CAR-T cell therapy

Extended Data Fig. 5

(a) Representative mass cytometry (CyTOF) plots illustrating the gating strategy for identifying CAR-T cells and T cell subsets as shown in Figs. 1a, 1d and Extended Data Figure 5e. (b) UMAP representation of peripheral blood mononuclear cells (PBMC) collected at time points shown in (a) shows the clustering of major immune cell types. (c) Relative contribution of major immune cell types in samples at different time points. (d) Expression of canonical markers, showing accurate classification of major immune celI types. (e) CAR-T cell phenotype, as determined by expression of CCR7 and CD45RA, illustrating a high fraction of effector-memory T cells at all time points. Each bar corresponds to N = 1 sample collected from the patient. The UMAP plots visually illustrate the clustering of T cells and confirm low CCR7 and CD45RA expression on CAR-T cells.

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