Extended Data Fig. 5: In-situ validation of FIKL cells at different developmental stages.
From: Insulin is expressed by enteroendocrine cells during human fetal development
a, Intestinal section with a typical example of an INS + cell marked with INS protein (green) from a 12 weeks GA fetus. Scale bar - 50 µm. b, Blowup of the INS + cell from (a) stained with both INS-mRNA (magenta) and INS-protein (green). Scale bar - 5 µm. c-f, Representatives examples of INS + cells in small intestine tissues from 12 weeks GA (c, 3 cells out of 20 found), 14 weeks GA (d, 2 cells out of 3 found), 18 weeks GA (e, 1 cell out of 1 found) and 21 weeks GA (f, 3 cells out of 23 found) stained with INS-mRNA (magenta), INS-protein (green). Scale bar - 5 µm. In all images nuclei are stained by DAPI (blue).
