Extended Data Fig. 6: Insulin is not detected in embryonic mouse small intestine.
From: Insulin is expressed by enteroendocrine cells during human fetal development
a, Mouse embryo intestinal section (E16.5) stained with the pan-enteroendocrine cell marker Chga (gray) and blowups of selected Chga+ cells. Scalebar - 50 µm for big image and 10 µm for blowups. b, Mouse embryo pancreatic tissue stained with Ins2-mRNA (magenta) and INS-protein (green) shown as a positive control for the in-situ detection of mouse cells expressing insulin. Scale bar - 10 µm. In all images nuclei are stained by DAPI (blue). Images are representative of 6 E16.5 embryonic subjects and 5 P0 neonatal subjects.
