Extended Data Fig. 7: MDS HSCs maintain the BM clonal burden in MDS-like mice during HMA therapy.

(a) Normalized CD45.2⁺ chimerism rates in HSCs (left) and MyHPCs (right) from mice competitively transplanted with CD45.1⁺ WT and CD45.2⁺ Vav-Cre or Vav-Cre/Tet2^L/L (Tet2^L/L) cells after two cycles of treatment with 5-aza (n=11 and n=13, respectively) or vehicle (n=13 and n=10, respectively). Lines represent the means ± s.d. of two independent experiments. No statistically significant differences were detected by one-way ANOVAs and Tukey’s multiple comparison tests for each cell type. (b) Normalized CD45.2⁺ chimerism frequencies in HSCs (left) and MyHPCs (right) from mice competitively transplanted with CD45.1⁺ WT and CD45.2⁺ Mx1-Cre or Mx1-Cre/Srsf2^P95H (Srsf2^P95H) cells after two cycles of treatment with 5-aza (n=12 and n=11, respectively) or vehicle (n=9 and n=12, respectively). Lines represent the means ± s.d. of two independent experiments. No statistically significant differences were detected using one-way ANOVAs and Tukey’s multiple comparison tests for each cell type. (c) Normalized CD45.2⁺ chimerism frequencies in HSCs (left) and MyHPCs (right) from mice competitively transplanted with CD45.1⁺ WT and CD45.2⁺ U2AF1^wt/rtTA or U2AF1^S34F/rtTA cells after two cycles of treatment with 5-aza (n=8/each) or vehicle (n=6 and n=5, respectively) in the presence of doxycycline. Lines represent the means ± s.d. of one experiment. No statistically significant differences were detected using one-way ANOVAs and Tukey’s multiple comparison tests for each cell type. (d) Normalized CD45.2⁺ chimerism frequencies in HSCs (left) and MyHPCs (right) from mice competitively transplanted with CD45.1⁺ WT and CD45.2⁺ Mx1-Cre or Mx1-Cre/Runx1^L/+/Srsf2^P95H (Runx1^L/+/Srsf2^P95H) cells after two cycles of treatment with 5-aza (n=5 and n=7, respectively) or vehicle (n=6 and n=7, respectively). Lines represent the means ± s.d. of one experiment. No statistically significant differences were detected using one-way ANOVAs and Tukey’s multiple comparison tests for each cell type.

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