Extended Data Fig. 7: Comparison of infection neutralization capacities for VoCs in twice BNT162b2 vaccinated individuals after breakthrough infection with delta and omicron compared to naive individuals and SARS-CoV-2 convalescents.

a, serum IC₅₀ values for infection-neutralization capacity normalized to 10⁷ viral RNA copies of SARS-CoV-2 variants in 47 naive participants (42 for omicron) 2 weeks after vaccination #2 (dark brown), 59 naive (light blue) and 36 convalescent participants 2 weeks after vaccination #3, as well as 12 and 10 twice BNT162b2 vaccinated individuals on average 7 days after PCR-confirmed breakthrough infections with delta (green) or omicron (purple), respectively; naives 2w after vaccination (vacc.) #2 vs. naives and convalescents 2w after vacc. #3 ^****P<0.0001 for all variants, vs. delta breakthrough infection for alpha ^**P=0.0043, gamma ^*P=0.0366, omicron ^**P=0.0074, vs. omicron breakthrough for alpha ^*P=0.0100, beta ^*P = 0.0246, gamma ^*P=0.0165, delta ^*P=0.0167, omicron ^***P=0.0007, convalescent 2w vacc. #2 vs. delta breakthrough for EU1 ^*P=0.0453, beta ^*P=0.0306, gamma ^*P=0.0230, delta ^*P=0.0226, omicron ^*P=0.0434. (b) IgG-type anti-spike antibody avidities in 44 naive participants 2 weeks after vaccination #2 (dark brown), 19 naive (light blue) and 18 convalescent participants 2 weeks after vaccination #3, as well as 10 and 8 twice BNT162b vaccinated individuals on average 7 days after PCR-confirmed breakthrough infections with delta (green) or omicron (purple), respectively; naives 2w vacc. #2 vs. naives and convalescents 2w after vacc. #3 ^****P<0.0001, vs. delta breakthrough ^***P=0.0001, and vs. omicron breakthrough ^**P=0.0014. (c) IgG-type anti-spike antibody avidities in twice BNT162b2 vaccinated individuals on average 7 days (n=10), 2 weeks (n=11), 3 weeks (n=7), and 4 weeks (n=8) after PCR-confirmed breakthrough infections with delta. Data are shown as box plots with median, bounds between upper and lower quartiles, and whiskers between the 10^th and 90^th percentiles. Differences between groups were analysed for their statistical significance using the Kruskal-Wallis test with Dunn’s multiple testing correction. Connecting lines indicate statistically significant differences between groups. Absence of connecting lines or asterisks indicates absence of significance. Abbreviations, inf: after infection; #2 – second vaccination; #3 – third vaccination; w1 – 7 days after infection; w2 – two weeks after respective vaccination/infection; w3 – three weeks after infection; w4 – four weeks after infection.

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