Extended Data Fig. 8: Sample quantification with combined Cas12 and Cas13 detection. | Nature Medicine

Extended Data Fig. 8: Sample quantification with combined Cas12 and Cas13 detection.

From: Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants

Extended Data Fig. 8

a, Kinetic curves of SARS-CoV-2 detection by both Cas13 (blue) and Cas12 (orange) for 5 or 7 concentrations, respectively, up to 2 h post-reaction initiation. Dashed lines represent the time at which signal reaches 50% (IC50 infection point) for each concentration of target. Error bars represent the 95% confidence intervals around the median fluorescence values (n = 2). b, Standard curves for SARS-CoV-2 and FLUAV based on RT-qPCR Ct values. Data related to Fig. 4c, now showing a standard 10-fold dilution series. Error bars represent the 95% confidence intervals around the mean RT-qPCR Ct (n = 6).

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