Fig. 6: Rapid and specific identification of the Omicron variant using the mCARMEN VIP. | Nature Medicine

Fig. 6: Rapid and specific identification of the Omicron variant using the mCARMEN VIP.

From: Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants

Fig. 6

a, Expected mutations across six SARS-CoV-2 variant lineages (Alpha, Beta, Gamma, Delta, Epsilon, Omicron) detectable by VIP. The blue boxes represent the presence of a mutation; the white boxes represent the absence of a mutation. b, Proportion of Omicron and Delta variant lineages as assessed by VIP in specimens (n = 430) collected on 6–16 December at HUCL. Green: Omicron; black: Delta. The error bars represent binomial sampling 95% confidence intervals (CIs). c, Proportion of Omicron and Delta variant lineages as assessed by VIP and NGS in specimens (n = 1,557) collected on 13–22 December from throughout the state of Massachusetts. Blue: Omicron; black: Delta; closed circles: mCARMEN; open circles: NGS. The error bars represent binomial sampling 95% CIs. d, Scatter plot of the proportion of Omicron based on the VIP and NGS variant lineage results. The linear regression line fit is shown as a blue line; R2 = 0.998. e, Comparison of the time delay from specimen extraction to determination of the variant lineage for VIP and NGS. The data represent the proportion of Omicron from specimens collected at HUCL and within the state of Massachusetts. Blue closed circles: VIP Massachusetts specimens from c; blue open circles: NGS Massachusetts specimens from c; green: VIP HUCL specimens from b.

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