Extended Data Fig. 7: Comparison of CD4bs bNAb and bNAb combination neutralizing activity from this study to other studies.
(a) CD4bs bNAb serum ID80 titers at baseline and rebound from each participant across the 4 studies3,4,5. Similar to Fig. 4b, see methods for ID80 calculation details. A one-sided Wilcoxon rank sum test was used to calculate statistical significance of differences between baseline and rebound titers for each patient, and p-values are shown on the top of the panel. All participants except 9341, 2C1 and 2E1, showed significantly lower serum ID80 titers at rebound as compared to baseline. (b) CD4bs bNAb IC80 values (µg/ml) as tested in vitro against baseline and rebound viruses from each participant across the studies. Similar to (A). Most participants showed significant difference (p < 0.05 using one-sided Wilcoxon rank sum test), with the exception of participants 693-7989, 91C22, 91C34, 91C35, 9342, 2C1 and 2E1 for whom no significant differences were found (p > 0.05). (c) Summary of in vitro IC80 values (µg/ml) of rebound viruses per study. Each point shows the per-participant median CD4bs bNAb IC80 value (µg/ml) for rebound viruses from each study. The only significant difference using two-sided Wilcoxon rank sum test was found to be between 3BNC117 + 10-1074 and VRC01 studies (p = 0.0134), and a trend when comparing this study to VRC01 (p = 0.0719); all other comparisons resulted in p > 0.17 (p-values not shown). (d) Same approach as (A) but using bNAb combination serum ID80 titers (see methods for combination ID80 titer calculation details). All participants showed significantly lower ID80 titers (that is due to higher resistance) for rebound viruses as compared to baseline viruses (p < 0.05 using one-sided Wilcoxon rank sum test). (e) Same approach (C) but summarizing the combination serum ID80 titers at rebound across studies. For 3BNC117 and VRC01 groups, single bNAb rebound ID80 titers are shown. The only significant difference was observed between the 3BNC117 + 10-1074 and VRC01 studies, with the former having significantly higher rebound ID80 titers than the latter (p = 0.0353 using two-sided Wilcoxon rank sum test). All other comparisons were not significant (p > 0.23; p-values not shown).
