Extended Data Fig. 4: Biological and genomic correlates of AML hierarchies.

A) Correlation between deconvoluted abundance of leukemic and immune cell types with clinical features in TCGA (n = 173). Only correlations with P < 0.05 are depicted, and correlations with FDR < 0.05 are noted with an asterisk. B) Cellular hierarchy projections of patient samples classified as FAB M0 (n = 30), M1 (n = 122), M2 (n = 77), M3 (n = 30), M4 (n = 58), M4Eo (n = 22), M5A (n = 28), or M5B (n = 11) C) Density plots depicting all mutation combinations along the Primitive versus Mature axis (PC2). D) Density plots depicting all mutation combinations along the Primitive versus GMP axis (PC1). E) Density plots depicting all cytogenetic alterations along the Primitive versus Mature axis (PC2). F-G) Impact of DNMT3A R882 mutations compared to other DNMT3A mutations on leukemic hierarchy organization along the Primitive versus Mature axis (PC2). F) Boxplot comparing PC2 of AMLs with DNMT3A R882 mutations (n = 84) compared to other DNMT3A (n = 96) mutations, split by mutational partner. Box plots indicate the range of the central 50% of the data, with the central line marking the median. Whiskers extend from each box to 1.5*(interquartile range). Statistical significance was evaluated through a two-sided Wilcoxon rank sum test. G) Density plot depicting PC2 of mutational combinations with either DNMT3A R882 or other DNMT3A mutations.