Extended Data Fig. 9: scRNA-seq analysis of microglial clusters from LV-injected 5xFAD mice.
From: Anti-inflammatory clearance of amyloid-β by a chimeric Gas6 fusion protein
a, Violin plots showing the expression levels of representative marker genes for the individual cell types from the 5xFAD hippocampus. b, Cluster fractions of total brain cells of the 5xFAD hippocampus injected with LV-Control, LV-Aducanumab, and LV-αAβ-Gas6. c, Individual UMAP plots of each treatment group. d, Heat map of 3 distinct microglia clusters characterized by suggestive plaque-associated microglia gene sets. e, UMAP clustering of the five microglial signatures (Homeostatic, DAM-like, INF, MHC-II, and Cyc-M) with defined microglial gene modules. The microglial gene signatures were calculated and scored using AddModuleScore function within the Seurat package. f, The bar graph showing the numbers of INF cluster cells in the 5xFAD hippocampus injected with LV-Control, LV-Aducanumab, and LV-αAβ-Gas6. g, Gene Ontology terms showing upregulated cellular pathways in LV-Aducanumab and LV-αAβ-Gas6 injected 5xFAD mice. These pathways were selected by 5 top score of each group after filtering by adjusted p-value <0.05.
