Extended Data Fig. 10: Bulk RNA-seq data showing distinct inflammatory responses of THP-1Axl by E2 and αFITC-Gas6 (E)-mediated phagocytosis.
From: Anti-inflammatory clearance of amyloid-β by a chimeric Gas6 fusion protein
a, Principal Component Analysis (PCA) showing clustering of each group: beads only (Control), beads treated with E2 (E2), and beads treated with αFITC-Gas6 (E) in THP-1Axl. b, Corresponding volcano plots of control versus E2 (left) and control versus αFITC-Gas6 (E) (right). The left plot showed 195 upregulated (log2FC >1, FDR <0.05) and 96 downregulated (log2FC < -1, FDR <0.05) genes, and the right plot showed 157 upregulated (log2FC >1, FDR <0.05) and 104 downregulated (log2FC < -1, FDR <0.05) genes. FC, fold change. c, Scatter plot for the fold change values of shared DEGs in Control versus E2 (y axis) and Control versus αFITC-Gas6 (E) (x axis). d, Gene set enrichment analysis (GSEA) revealed 195 gene-sets and 157 gene-sets enriched for Control versus E2 and Control versus αFITC-Gas6, respectively. Selected pathway filtered by adjusted p-value <0.05. e-f, Gene-set enrichment analysis (GSEA) comparing E2 or αFITC-Gas6 (E). Gene-set terms are indicated as followed; FDR, false discovery rate; NES, normalized enrichment score.
