Extended Data Fig. 1: Characterization of TAM receptor expression in HMC3 cells and target binding mediated by Gas6 fusion proteins.
From: Anti-inflammatory clearance of amyloid-β by a chimeric Gas6 fusion protein
a, mRNA expression levels of TAM and Fc gamma receptors measured by qRT-PCR in HMC3 cells. Mean ± s.e.m. b, Immunoblot analysis for TAM receptor expression using the cell lysates of THP-1 and HMC3 cells. c, Surface expression of TAM and Fc gamma receptors in HMC3 cells analyzed by flow cytometry d, Schematic illustration showing target-specific interaction between beads and AXL receptor induced by Gas6 fusion proteins. e, Gating strategy for flow cytometry analysis to confirm binding of target beads to HMC3 cells. f, Flow cytometry histogram showing the binding of FITC- or oAβ-conjugated beads on HMC3 cells in the presence of different Gas6 fusion proteins. Substitution of the Gla domain with scFv sequence was detected by anti-scFv Fab. g, Flow cytometry analysis showing that Gas6 fusion proteins lost their ability to bind to apoptotic thymocytes generated by treatment with dexamethasone (12.5 μM) for 24 hr. For a, b, representative data from n = 3 biological replicates.
