Extended Data Fig. 4: Expression of TAM and Fc gamma receptors in primary rat glial cells.
From: Anti-inflammatory clearance of amyloid-β by a chimeric Gas6 fusion protein
a, Quantitative bar graphs showing the mRNA levels of Axl, Mer, Cd64, and Cd16 in cultured microglia and astrocytes measured by qRT-PCR at DIV 7. mRNA expression levels were normalized by Axl expression level in each glial cell. Mean ± s.e.m.; b, Immunoblot analysis for TAM receptor expression using the cell lysates of primary microglia and astrocytes. c, Amino acid sequence alignment of LG domains of human, rat, and mouse Gas6 proteins. LG domains of human Gas6 protein shares about 80 percent identity with those of rat or mouse Gas6. The asterisk (*, dark gray), colon (:, gray), and period (., light gray) indicate positions which have a single, fully conserved residue (*), conservation between groups of strongly similar properties (:) or weakly similar properties (.), respectively. d, Immunoprecipitation analysis for tyrosine phosphorylation of AXL in primary microglia or astrocytes. Target cells were incubated with oAβ (2.5 μM) or along with αAβ-Gas6 (4 μg/mL) for 15 min. NT, non-treated. e, Representative Incucyte images of primary microglia (top) and astrocytes (bottom) engulfing oAβ-pHrodo upon treatment of αAβ-Gas6 (5μg/mL) with αTYRO3 (10 μg/mL), αAXL (10μg/mL), or αMERTK (30 μg/mL) antibodies. Scale bar = 100 μm. Mean ± s.e.m. For a, b, d, e, representative data from n = 3 biological replicates.
