Extended Data Fig. 2: Additional insights into the genomic landscape of rectal cancer.

(a) Overview of driver alterations in rectal cancer stratified by tumor stage. (b) Distribution of clonal versus driver mutations for the most frequently mutated genes in our rectal cancer cohort. (c) Fraction of samples with two driver mutations in selected genes where both are clonal, both are subclonal or only one is clonal. (d) Distribution of KRAS mutations stratified by affected codon and specific amino acid change. Blue vertical bars show the fraction of clonal versus subclonal mutations. Red and gray bars show the fraction of samples with allelic imbalance (mutant selection). (e) Distribution of mutational signatures for samples in the WES cohort. Samples were ordered from left to right in terms of decreasing SBS1 signature (mitotic clock) and stratified according to dMMR/MSI status.