Extended Data Fig. 2: Cell product differentiation into astrocytes. | Nature Medicine

Extended Data Fig. 2: Cell product differentiation into astrocytes.

From: Transplantation of human neural progenitor cells secreting GDNF into the spinal cord of patients with ALS: a phase 1/2a trial

Extended Data Fig. 2

Cell product differentiation into astrocytes. Representative image and quantification of double immunofluorescence for (a, b) CNS10-NPC-GDNF labeled with Stem101 (nuclear specific, green) and human nestin, a neural progenitor marker, and with (c, d) GFAP, an astrocyte marker (red). (e) Double immunofluorescence shows that many cells are positive for human nestin and GFAP. (f) Confocal microscopy images show that CNS10-NPC-GDNF labeled with Stem 121 (cytoplasmic specific, red) co-localized with mature astrocyte markers aquaporin 4 (AQP4) and Glast (green). Nuclei counterstained with DAPI (blue). White arrows indicate CNS10-NPC-GDNF co-expressing the markers. Sample size n = 15 biologically independent animals for each dose and n = 10 for vehicle. Error bars ± s.e.m. Scale bars: (a, c) 10 µm; (e) = 75 µm; (f low mag) 10 µm; (f high mag) 5 µm.

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