Extended Data Fig. 3

(a-f) Linear discriminant analysis (LDA) scores for differentially abundant taxa in (a) response, (c) irAE or (e) ‘Benefical/benign’ and ‘Adverse’ outcome groupings, as determined by LEfSe analysis (Australian cohort n = 71). LDA score indicates the confidence of the association, p < 0.05 for the Kruskal-Wallis H statistic, LDA score >3. (b, d & f) Dot plots show the relative abundance of sequence reads corresponding to each taxa for individual patients, where each dot represents a patient, colours correspond to the legend as indicated. (g-h) Quantitative PCR using taxa specific primers was used to determine bacterial copy number per mg faecal matter grouped according to response, irAEs and ‘Adverse’ outcome groups (n = 71). (g) Taxa associated with response or mild irAE based off LefSe analysis (h) taxa associated with non-response or severe irAE. Each symbol represents an individual patient, bars indicate the median. Mann-Whitney U rank sum test (g-h). All statistical tests are two-sided where appropriate.