Extended Data Fig. 4

(a-c) Archaeal ASV in pre-treatment faecal samples were classified using 16 S rRNA gene sequencing. Relative abundance of Archaeal ASVs (Methanobacteriaceae) from 16 S rRNA amplicon sequencing was compared by response, maximum irAE grade and ‘Beneficial/benign’ vs ‘Adverse’ outcomes (n = 71). (d) Quantitative PCR on faecal DNA using methanogen specific primers, grouped by response (left) or maximum irAE grade (right) (‘Adverse’ patients are indicated in orange). Each symbol represents an individual patient, bars indicate the median. Mann-Whitney U rank sum test (a-d). All statistical tests are two-sided where appropriate.