Table 1 Patient and sample characteristics
Case | Time point | Sample type | Symptoms | MPXV PCR on leftover DNA extract (Ct value) | MPXV PCR on original sample (Ct value) | Orthopox virus PCR on leftover DNA extract (Ct value) | Whole-genome sequencing | Viral viability study | Orthopox virus IgG antibodies (titer) |
|---|---|---|---|---|---|---|---|---|---|
1 | Day 0 | Pooled samplea | None reported | Positive (27.63) | Anorectal swab: positive (26.69); oropharyngeal swab: negative | Positive (30.35) | ND | Negative | Negative (<1:20) |
Day 37 | Anorectal swab | None reported | NA | Negative | NA | NA | NA | Positive (1:320) | |
2 | Day 0 | Anorectal swab | None reported | Positive (22.25) | Positive (20.05) | Positive (23.50) | Yes | Positive | Negative (<1:20) |
Day 21 | Anorectal swab | None reported | NA | Negative | NA | NA | NA | Positive (1:40) | |
3 | Day 0 | Anorectal swab | None reported | Positive (19.19) | Positive (17.16) | Positive (21.43) | ND | Positive | Negative (<1:20) |
Day 24 | Anorectal swab | None reported | NA | Negative | NA | NA | NA | Positive (1:80) | |
4 | Day 0 | Anorectal swab | Painful vesicular perianal rash | Positive (29.06) | Positive (27.38) | Positive (28.3) | ND | Negative | NA |
