Extended Data Fig. 8: Correlation between ELISAs with VACV and MPXV antigen, and neutralization assays with MPXV grown on Vero or Calu-3 cells. | Nature Medicine

Extended Data Fig. 8: Correlation between ELISAs with VACV and MPXV antigen, and neutralization assays with MPXV grown on Vero or Calu-3 cells.

From: Low levels of monkeypox virus-neutralizing antibodies after MVA-BN vaccination in healthy individuals

Extended Data Fig. 8

(a) ELISAs were performed on the age-panel, diagnostic panel, and Imvanex panel of sera with a VACV-infected or MPXV-infected cell lysate. 30% endpoint ELISA titers were calculated based on a 5-fold dilution series, after subtraction of the OD450 values from mock-infected cell lysate from the OD450 values from VACV-infected or MPXV-infected cell lysates, and relative to a positive control. (b) PRNTs were performed with an MPXV stock grown on either Calu-3 or Vero cells. The 50% plaque reduction neutralization titer (PRNT50) was calculated on the basis of a 2-fold dilution series relative to an infection control. Sera were obtained from individuals born in or prior to (triangles), or after (circles) 1974, who were either PCR-negative (red and gray), PCR-positive (green), or vaccinated (blue).

Back to article page