Extended Data Fig. 5: Normalized S-curves for determining the 30% OD450 ELISA endpoint in MVA-H5 sera. | Nature Medicine

Extended Data Fig. 5: Normalized S-curves for determining the 30% OD450 ELISA endpoint in MVA-H5 sera.

From: Low levels of monkeypox virus-neutralizing antibodies after MVA-BN vaccination in healthy individuals

Extended Data Fig. 5

Sera from the MVA-H5 panel were 5-fold diluted starting at a dilution of 1:10 and added to plates coated with a VACV-infected cell lysate. A positive reference control was included on every plate to generate an S-curve with minimum (0%) and maximum (100%) OD450 values. All sample OD450 values were normalized to the positive control on the respective plate and plotted as log (inhibitor) vs response curve with four parameter variable slope using GraphPad Prism 9.02. A 30% endpoint dilution was estimated by calculating the proportionate distance between two dilutions, from which a titer was determined. Symbols indicate individual donors, cyan lines represent sera from MVA-H5-vaccinated donors, purple lines represent the controls from the different plates. 30% endpoint titers are shown in Fig. 2d. All samples from this serum panel were run in the same experiment, avoiding any possible batch effects.

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