Extended Data Fig. 7: Differentiation between MVA vaccination and MPXV infection by serology. | Nature Medicine

Extended Data Fig. 7: Differentiation between MVA vaccination and MPXV infection by serology.

From: Low levels of monkeypox virus-neutralizing antibodies after MVA-BN vaccination in healthy individuals

Extended Data Fig. 7

ELISAs were performed on sera from either vaccinated and MPXV PCR-positive individuals with a VACV-infected cell lysate. 30% endpoint ELISA titers were calculated based on a 5-fold dilution series, after subtraction of OD450 values with a mock-infected cell lysate, and relative to a positive control. PRNTs were performed with MVA or MPXV. The 50% plaque reduction neutralization titer (PRNT50) was calculated on the basis of a 2-fold dilution series relative to an infection control. (a, b) Correlation of VACV ELISA and MVA PRNT. (c, d) Correlation of VACV ELISA and MPX PRNT. (e, f) Correlation of MVA PRNT and MPXV PRNT. The bubbles in panels A, C, and E enclose all datapoints from the indicated samples set. The centers of bubbles from vaccinated individuals were connected by arrows to indicate antibody level increases, as indicated in panels B, D, and F. (G) 3D representation of the data presented in panels A, C, and E.

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