Extended Data Fig. 2: Computationally determined off-target sites for h403_sgRNA with ABEmax-VRQR.
From: Base editing correction of hypertrophic cardiomyopathy in human cardiomyocytes and humanized mice
a, Genomic loci of eight candidate off-target (OT) sites (left) and alignments of eight candidate off-target sequences to the on-target protospacer (right). Nucleotides that match the protospacer are indicated with a vertical dash. Nucleotides that differ are shown for each site. Numbering of nucleotides in protospacer starts with the nucleotide immediately 5′ of the PAM as nucleotide 1. b, HTS to measure editing for all 58 adenines within the protospacers of the top 8 CRISPOR-identified candidate off-target loci. HTS was performed for ABE-treated MYH7403/+ HCM1 and MYH7403/+ HCM2 iPSCs.
