Extended Data Fig. 2: Timeline of viral outgrowth assays.

a, Timeline of the viral outgrowth assays. Orange arrows indicate assays performed at the Heinrich Heine University Duesseldorf, green arrows indicate assays performed at the Leibniz Institute of Virology, and blue arrows assays at the IrsiCaixa AIDS Research Institute. All qVOA showed negative results and an in vivo viral rescue assay in Balb/c Rag2^−/−γc^−/− mice showed negative results in the immunohistochemical stainings for HIV-1 p24 Ag in the spleens, livers, and lymph nodes (Supplementary Fig. 1). HSCT, hematopoietic stem cell transplantation; ATI, analytical treatment interruption; qVOA, quantitative viral outgrowth assay; mVOA, murine viral outgrowth assay. b, A mVOA in n = 5 biologically independent NOD-scid mice from month 42 after HSCT showed good engraftment of the transferred CD4+ T cells and undetectable plasma HIV-1 RNA and cellular HIV-1 DNA in the blood of the mice. Filled symbols indicate detectable values and open symbols indicate the limit of detection for undetectable values. After six weeks, HIV-1 DNA in the spleen of the mice was undetectable when infused with the IciStem no. 19 patient cells (blue) compared to an HIV-1 positive control patient under ART (grey) (two-tailed Mann-Whitney test; P = 0.0079). Data are shown as mean with standard deviation.