Extended Data Fig. 1: Cough Aerosol Sampling System (CASS) used to measure culturable M. tuberculosis in cough aerosol droplets as a surrogate for infectiousness.

The CASS system consisting of a six-stage Anderson cascade impactor (ACI) showing the median expected droplet diameter (µm) for each stage (Panel A).Patient sitting in a negative pressure cubical while coughing into a mouth piece that is attached to the ACI (Panel B).Culture plate that enables CFUs from individual aerosol droplets to be isolated (Panel C) The ACI was horizontally contained in a 10 litre autoclavable chamber (panel D).