Extended Data Fig. 10: Analysis of the white matter signal in the RELN-COLBOS in comparisons to other AD cases.
From: Resilience to autosomal dominant Alzheimer’s disease in a Reelin-COLBOS heterozygous man
a, Representative images for luxol fast blue (LFB) staining (large panel and small left panels) and RELN-CT (small right panel) of the sub hippocampal white matter of the RELN-COLBOS case, the APOECh case, a representative PSEN1 E280A FAD case and a representative SAD case. Scale bars, 50 𝜇m. b, Bar graph for normalized measurement of white matter relative to total LFB signal of the RELN-COLBOS case, the APOECh case, PSEN1 E280A FAD cases (n = 5) and SAD cases (n = 4). Both the RELN-COLBOS case and the APOECh case show relative higher white matter signal than the other FAD cases. c, Bar graph for the quantification of RELN-CT signal in round particles in the sub-hippocampal white matter of the RELN-COLBOS case, the APOECh case, PSEN1 E280A FAD cases (n = 5) and SAD cases (n = 4). The RELN-COLBOS case shows higher RELN-CT signal intensity than all FAD cases, including the APOECh one. d, Line graph for RELN-CT positive round particles signal intensity distribution in the RELN-COLBOS case, the APOECh case, PSEN1 E280A FAD cases (n = 5) and SAD cases (n = 4). The RELN-COLBOS case showed a larger percentage of particles with higher RELN-CT signal intensity when compared with the other AD cases. e, Scatter plot of Spearman’s Rho correlation analysis for LFB signal intensity and RELN-CT round particles signal intensity in all analyzed AD cases. A statistically significant positive correlation was identified (p = 0.003, R = 0.818).
