Fig. 2: The colonic dysplasia landscape of PSC is enriched in the I2 signature and differs from that of IBD.

a–d, Patients with active right-sided dysplasia were analyzed (n = 6 with PSC, n = 7 with IBD, n = 8 sporadic). a, Distribution of individuals with right colon dysplasia across clusters; statistical significance was determined using a chi-squared test. b, Histologically scored inflammation at the site of dysplasia within the right colon using the UCM histological criteria for grading disease activity: 0, no diagnostic abnormality; 1, quiescent or minimally active; 2, mild; 3, moderate; and 4, severe. The right colon includes the cecum, ascending colon and hepatic flexure. Significance was determined using a double-sided Wilcoxon rank-sum test. c, Single-sample gene set enrichment analysis (GSEA) inflammatory response score calculated from the transcriptome of the right colon tissue biopsy. Significance was determined using a double-sided Wilcoxon rank-sum test without adjustment for multiple comparisons. The center line represents the median; the hinges indicate the first and third quartiles; the upper and lower whiskers extend to the largest and smallest values that are within 1.5 times the interquartile range (IQR) from the first and third quartiles, respectively (b,c). d, Bar graph quantifying the percentage of differentially expressed genes (DEGs) (adjusted P < 0.05) in each comparison (the proportion of genes upregulated in PSC are shown in purple, genes upregulated in sporadic dysplasia are shown in green, and genes upregulated in IBD are shown in orange).