Extended Data Fig. 4: General flow-based immunophenotyping.
From: Transcriptional signatures associated with persisting CD19 CAR-T cells in children with leukemia
(a) Immunophenotyping non-CAR and CAR-T cells by multi-parameter flow cytometry. All cells were initially gated for CD3 and the CAR. Red boxes and arrows highlight subsequent gating strategies for representative examples illustrating double-negative CAR-T cells are αβ T cells and CD8A/CD8B negative, in contrast to non-CAR-T cells. Fluorochromes: PerCP-Cy5.5 = Peridinin chlorophyll protein-Cyanine5.5, FITC = Fluorescein isothiocyanate, Brilliant Violet (BV) 650, BV510, BV786. (b) Trajectory scatter plots quantify flow plots signals across patient samples for CD45RA and CCR7. Cells were gated for CD3 and the CAR. TN = Naïve, TSCM = Stem Cell Memory, TCM = Central Memory, TEM = Effector Memory, TEMRA = Terminally Differentiated Effector Memory expressing CD45RA.
