Extended Data Fig. 5: Gamma-Delta and NK Immunophenotyping CAR-T cells by multi-parameter flow cytometry. | Nature Medicine

Extended Data Fig. 5: Gamma-Delta and NK Immunophenotyping CAR-T cells by multi-parameter flow cytometry.

From: Transcriptional signatures associated with persisting CD19 CAR-T cells in children with leukemia

Extended Data Fig. 5

All cells were initially gated for CD3, CAR, CD4 and CD8A. (a) Flow plots for representative samples (P06 and P09) with high populations of double-negative TCRγδ cells. (b) Trajectory scatter plots quantify flow plots signals across patient samples for TCRγδ. Cells were previously gated for CD3, CAR, CD4 and CD8. (c) Flow plots show gating for CD16 and CD56. Prior gating includes CD3, CAR, CD4, CD8. Fluorochromes: APC = Allophycocyanin, Cy = Cyanin.

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