Extended Data Fig. 8: Clonal structure of CAR-T cells.

(a) Clonal trajectory illustrates the changes in clonal architecture of CAR-T cells over time across a second representative patients with more than 3 timepoints sequenced. Packed circle plots show the size of each clonotype. Filled-in black circles represent clonotypes that are not unique, as they are observed across timepoints. Conversely, pink donut circles represent clonotypes that are unique to that timepoint (and not observed across time). Blue/purple/grey coloured circles represent the dominant clonotype at that timepoint that correspond with the clonal trajectories below. (b) Cell type composition stacked bar plots demonstrate the shift in cell type abundances between early timepoints and late timepoints between unique and non-unique clonotypes (right). (c) Trajectory scatter plots quantify data from all patient samples for CAR expression across time.