Fig. 2: Characterization of CARPALL CAR-T cells.
From: Transcriptional signatures associated with persisting CD19 CAR-T cells in children with leukemia
a, UMAP of CAT CAR-T cells demonstrates transcriptional clustering based on timepoint. Product = infusion products (M0); early = M1–M3; mid = M4–M6; late = M7–M60. b, UMAPs show scaled average expression of lymphocyte markers. c, Immunophenotyping CAT CAR-T cells by multi-parameter flow cytometry. Representative example of P01 showing cell identification using CD4 and CD8A at an early (M2) and a late (M72) timepoint. Cells were gated for CD3 and CAT CAR. d–f, Trajectory scatter plots quantify data from all patient samples for CD4, CD8 and double-negative CAR-T populations. g, Representative example of P01 showing immunophenotyping using CD45RA and CCR7 at an early (M2) and a late (M72) timepoint. Cells were gated for CD3 and CAT CAR. TCM, central memory; TEM, effector memory; TEMRA, terminally differentiated effector memory expressing CD45RA; TN, naive; TSCM, stem cell memory. Fluorochromes: BV, brilliant violet; Cy, cyanine dye; PE, R-phycoerythrin; PerCP, peridinin-chlorophyll protein.
