Extended Data Fig. 3: Semi-automated workflow for CD8-positive cell quantitation in tumour biopsies. | Nature Medicine

Extended Data Fig. 3: Semi-automated workflow for CD8-positive cell quantitation in tumour biopsies.

From: Ipilimumab with or without nivolumab in PD-1 or PD-L1 blockade refractory metastatic melanoma: a randomized phase 2 trial

Extended Data Fig. 3

a) Representative images for manual annotation of tumour and tumour periphery regions. b) Screenshot of cell segmentation, identifying CD8-positive cells (red) and CD8-negative cells (blue), for CD8 cell quantitation. Scale bars in each panel are 100um. c) Density of CD8 positive cells detected within the annotated tumour periphery. Paired baseline and on-treatment biopsies are indicated by points connected by lines. Dotted lines indicate patients with pathological features of regressed melanoma in the on-treatment biopsy. The number of patients shown in each group is indicated by the number along the x-axis (Ipilimumab arm, N = 2 partial response [PR], N = 3 stable disease [SD], N = 13 progressive disease [PD]; nivolumab and ipilimumab arm, N = 7 complete response [CR], N = 8 PR, N = 7SD, N = 25 PD). Box plots indicate the median (middle line), 25th and 75th percentiles (box) and 5th and 95th percentiles (whiskers).

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