Extended Data Fig. 8: Diagnosis-related expression of GAL3 in the human cerebral cortex.
From: Injury-specific factors in the cerebrospinal fluid regulate astrocyte plasticity in the human brain
(a-c) Appearance of GAL3 (brown color) in individual autopsy samples of the cerebral cortex parenchyma from patients with stroke (a,a′, a′′), COVID-19 (b, b′, b′′), as well as in biopsy samples of astrocytoma (c, c′, c′′). Examples of the affected cerebral GM areas (left panels in a-c) are shown at higher magnifications (right panels in a-c). (d) Representative maximum projection image showing examples for GFAP, GAL3 and MIB1 immunolabelling in astrocytoma biopsy (e-g). Notably, GFAP+MIB1+ cells in tumor adjacent tissue are GAL3−(yellow arrowheads in e′ and e′′), while proliferating (MIB1+) cells at the pathological tumor vessels express GAL3 (f, g). n = 3 patients per diagnosis. GM: cerebral grey matter. Cell nuclei were counterstained with hematoxylin (in a-c) or DAPI (in d-g). Scale bars: 150 µm (a-c), 75 µm (d, e), 50 µm (f), 20 µm (g).
