Extended Data Fig. 3: Conserved and distinct signatures of tissue resident myeloid cells in CNS interfaces between mouse and man.
From: Multiomic spatial landscape of innate immune cells at human central nervous system borders
a. Heatmap visualization of latent factors determined by multiomic factor analysis (MOFA)2 analysis of macrophages across the indicated compartments. The color scale indicates the percentage of variance explained across the compartments. For enhanced readability, the respective values are indicated in each heat map tile. Selected top genes for each latent factor are presented to the right of the heat map. PC/PV: parenchyma/perivascular space, CP: choroid plexus, LM: leptomeninges, DM: dura mater. b. Dotplot showing gene ontology term enrichment analysis across the top 100 differentially enriched genes in the MOFA2 latent factors from panel a. The dot size indicates the gene ratio of genes differentially expressed in each cluster over the genes in the indicated gene ontology terms. The color coding of the dot indicates the Benjamini–Hochberg adjusted p value based on a one-sided Fisher’s exact test.c. Dot plot depicting the cross-species comparison of differentially expressed orthologue genes between CAMs and microglia in humans and mice. On the x-axis, adjusted average log2-fold changes from the comparison of murine MHC-IIlow and MHC-IIhigh are signed positively if they are upregulated in the former and negatively if they are upregulated in the latter. The y-axis contains the adjusted average log2-fold changes of the human counterparts. The log2-fold changes were calculated using two-sided unpaired Wilcoxon Rank-Sum tests followed by Bonferroni correction for multiple testing. Orthologue genes in the top right and bottom left quadrants are differentially expressed in the same direction. Orthologue genes in the top left and bottom right quadrants are showing opposite directions of differential expression. Orthologue genes positioned along the axes are only upregulated in one species. The color coding indicates the directionality of the gene expression across species. Blue genes were upregulated in mice and humans; red genes were downregulated in mice and humans; green genes were upregulated in humans and downregulated in mice; violet genes were differentially regulated in humans, but not in mice; orange genes were differentially regulated in mice but not in humans.
