Table 2 No effect of subclinical atherosclerosis on the expansion of mutant hematopoietic clones

	AER			log (VAF ratio)/time			Exponential growth rate
	Estimate	Standard error	P value	Estimate	Standard error	P value	Estimate	Standard error	P value
Baseline presence of atherosclerosis
CACS >0	−1.907	1.474	0.196	−1.161	0.893	0.194	−1.266	0.94	0.179
Global plaque volume >0	0.718	1.183	0.544	0.034	0.734	0.963	0.075	0.772	0.923
Carotid plaque volume >0	0.526	1.321	0.691	−0.071	0.798	0.929	−0.016	0.841	0.985
Femoral plaque volume >0	1.012	1.287	0.432	0.155	0.803	0.847	0.205	0.843	0.808
Baseline extent of atherosclerosis
CACS (Agatston)	0.003	0.005	0.524	0.003	0.003	0.376	0.003	0.003	0.392
Global plaque volume (mm³)	−0.007	0.007	0.304	-0.005	0.004	0.2	−0.005	0.004	0.214
Carotid plaque volume (mm³)	−0.019	0.025	0.46	−0.005	0.014	0.742	−0.006	0.015	0.695
Femoral plaque volume (mm³)	−0.013	0.008	0.127	-0.009	0.005	0.061	−0.009	0.005	0.069
Cumulative atherosclerosis burden over 6 years
CACS (AUC)	1.816	10.08	0.857	3.044	6.121	0.619	2.867	6.444	0.657
Global plaque volume (AUC)	−7.095	6.955	0.308	−4.934	4.305	0.252	−5.38	4.53	0.236
Carotid plaque volume (AUC)	−9.817	14.13	0.488	−4.569	8.526	0.592	−5.268	8.99	0.558
Femoral plaque volume (AUC)	−11.047	8.223	0.18	−9.13	5.111	0.075	−9.53	5.371	0.077

We monitored the expansion of 602 CH-related mutations through serial sequencing of blood samples from 494 individuals at baseline and ∼6 years after enrollment. We then investigated the association between baseline or cumulative atherosclerosis burden and different metrics of clonal expansion rates through mixed-effects models. The presence of subclinical atherosclerosis at baseline was defined as a categorical variable on the basis of CACS or 3DVUS imaging. The extent of subclinical atherosclerosis at baseline in those with detectable atherosclerosis in specific regions was defined as a continuous variable on the basis of CACS or 3DVUS imaging. The cumulative burden of subclinical atherosclerosis over the timeframe of this longitudinal analysis was estimated as the AUC for CACS and atherosclerotic plaque volumes, assessed by CT and 3DVUS imaging, respectively, at three different time points over 6 years.

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