Extended Data Fig. 4: CART-123 dysfunction caused by inability to clear AML antigen.

a, Flow cytometry analysis of expression of additional inhibitory receptors CD38 and CD39 (top) and CTLA4 and CD94 (bottom). Representative of two experiments with different primary AML and CART-123 donors, complements distinct donors shown in Fig. 3. b, Intracellular expression of TCF-1 in the indicated CART-123 + T cell subsets, measured by flow cytometry. MFI displayed to the right. Representative of two experiments with different primary AML and CART-123 donors complements distinct donors shown in Fig. 3. See also, Supplementary Fig. 3. c, Flow cytometry analysis of live single cells depicting residual CD3- (presumed AML cells) after 2 weeks of co-culture. d, Intracellular cytokine analysis of IFN\(\gamma\) and TNF\(a\) in two different CART-123 donors after 3 weeks of UPA or CPA co-culture. e, Surface expression of inhibitory receptor ligands on two distinct primary AML samples after one week in co-culture with CART-123 cells. f, UMAP plots of SCFV-expressing, CAR + T cells recovered from patients at time points at least 10 days after infusion. On the left coloration is determined by patient response, and on the subsequent 3 the indicated “gene module score” is overlaid.