Fig. 1: Overview of multi-organ vascular cell atlas. | Nature Medicine

Fig. 1: Overview of multi-organ vascular cell atlas.

From: An organotypic atlas of human vascular cells

Fig. 1

a, Organ and tissue single-cell RNA-seq datasets used for analysis. b, UMAP representation of all cell types from the global integrated object, including vascular and non-vascular types. c, Dot plot representation of selected EC and mural cell marker genes and markers of other major cell types identified in the global cell type object. d,e, smFISH for TINAGL1 (scale bar, 50 μm) in ileum and skeletal muscle with nuclear counterstain (DAPI) (d) and quantification of TINAGL1 co-expression with ACTA2 (VSMC) and CDH5 (EC) (n = 3 regions of interest (ROIs) × 3 donors) (e). Statistical analysis was performed using the Wilcoxon rank-sum test with Benjamini–Hochberg adjustment. * adjusted P < 0.05; *** adjusted P < 0.001. Error bars indicate standard error. f, UMAP representation of broad vascular cell types subset from the global integrated object. g, Dot plot representation of selected marker genes for major cell states within the vascular compartment. h, Dendrogram of hierarchical clustering of EC populations subset per organ. Top color bar: organ. Bottom color bar: EC subtype. The illustration in a was created with BioRender.

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