Extended Data Fig. 8: Spatial validation of interacting cell pairs in the heart. | Nature Medicine

Extended Data Fig. 8: Spatial validation of interacting cell pairs in the heart.

From: An organotypic atlas of human vascular cells

Extended Data Fig. 8

a, Approach to obtaining relevant cell-cell interactions using non-negative matrix factorisation (NMF) coupled with manual annotation of spatial transcriptomic data. b, NMF analysis demonstrating cellular microenvironments. Colour density = loading of the corresponding cell type for the specific NMF factor. c & d, Example of manual annotation of arteries (c) and veins (d) using heart spatial transcriptomics data. e & f, Odds ratio analysis of enriched cell types within manually annotated arteries (e) and veins (f). Significant cell type enrichment is highlighted in green, with p values < 0.05. Error bars in (e) and (f) indicate the 10% confidence interval. The illustration in (a) was created with BioRender.com.

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