Extended Data Fig. 3: Characterisation of arterial, endocardial and capillary EC subpopulations.

a, smFISH staining for SULF1 (red - top panel) and b, ELN (magenta - bottom panel) with SEMA3G (yellow) in arterial EC. Nuclei were counterstained with DAPI. Scale bars = 40 μm c, UMAP representation of individual arterial EC populations after integration for trajectory inference (as shown in Fig. 2h). d, UMAP representation of SULF1 (left) and NEBL (right) gene expression on integrated object used for trajectory inference. Scale bar represents log1p transformed gene expression values. e & f, UMAPs (left) show specific zonation signatures, and heatmaps (right) represent differentially expressed genes determined within specific zonation segments, including aorta_coronary_ec to art_ec_1 (e) and art_ec_1 to art_ec_2 (f). g, Regulon plots showing the SCENIC ranking of transcription factors for endocardial ECs with the top 5 transcription factors highlighted.