Extended Data Fig. 4: Image preprocessing of mouse kidney H-scans.
From: A first-in-human study of quantitative ultrasound to assess transplant kidney fibrosis

(a) Normal kidneys which did not contain hypoechoic inner regions were first manually contoured. Morphological erosion was then used to separate out the outer and inner regions of the kidney. All three masks were used to perform H-scan analysis on the whole kidney, the cortex (outer region), and the medulla (inner region). (b) Mouse kidneys which contained inner hypoechoic regions were run through morphological opening to exclude these regions before erosion separated the cortex from the medulla. In addition, contouring was also used to segment the entire kidney. Created with BioRender.com.